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Wet Lab: DNA Barcoding: From Samples to Sequences

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Portable Document Format
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Description This is a lab activity outlined by a PDF and accompanied by a PowerPoint presentation to be shown to students. Students perform the wet lab experiments necessary for DNA barcoding. Students will purify DNA, perform polymerase chain reaction (PCR), and analyze the PCR products by agarose gel electrophoresis. This resource is listed at the bottom of the lessons on the URL referenced, and titled "Wet Lab: DNA Barcoding: From Samples to Sequences."
Type of Resource Diagram, Digital Presentation (Powerpoint), Illustration, Laboratory or Hands-On Activity
Format Portable Document Format - PDF
Technical Note -This resource is listed at the bottom of the lessons on this page. Titled: "Wet Lab: DNA Barcoding: From Samples to Sequences" -Adobe Acrobat Reader -Microsoft PowerPoint
Jodie Spitze, Kent Meridian High School
Joan Griswold, Northwest Association for Biomedical Research
Dina Kovarik, Northwest Association for Biomedical Research
Sandra Porter, Digital World Biology
Karen Peterson, EdLab Group
Development Date April 1, 2011
Grade/Age Levels High School lower division (Grades 9-10)
High School upper division (Grades 11-12)
Undergraduate lower division (Grades 13-14)
National Science
Educational Standards
Abilities necessary to do scientific inquiry (K-12), Understanding about science and technology (K-12), Understandings about scientific inquiry (K-12)
Learning Time 4-6 hours
Language English
Type of Review Reviewed by Partner Organization
Review Date July 24, 2011
Funding Source National Science Foundation
Suggested Use
This resources includes the instructions for DNA sequencing from DNA extraction to base by base sequencing. There is a power-point which corresponds to each step of the process. I have not used this in the classroom, but as a former lab technician, I found the resource to follow the process very well. There are instructions for each step of the lab and a timeline to follow.
Raena Cota, New Mexico State University


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